MOTS-c is a synthetic peptide derived from the mitochondrial genome, designed for in vitro and animal model research to investigate cellular metabolism and mitochondrial signaling pathways. It is utilized in laboratory studies to explore its role in regulating energy homeostasis, insulin sensitivity, and mitochondrial function in cell lines and rodent models under controlled conditions. Researchers employ MOTS-c to assess its interactions with metabolic pathways and nuclear gene expression, focusing on its potential to modulate cellular energy production and stress responses. Analytical techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry are used to confirm its structural integrity and purity for experimental applications. This peptide is intended exclusively for scientific investigations to advance understanding of mitochondrial-derived signaling in experimental research settings.